Engineering the DNA cytosine-5 methyltransferase reaction for sequence-specific labeling of DNA

Engineering the DNA cytosine-5 methyltransferase reaction for sequence-specific labeling of DNA

DNA methyltransferases catalyse the transfer of a methyl group from the ubiquitous cofactor S-adenosyl-L-methionine (AdoMet) onto specific target sites on DNA and play important roles in organisms from bacteria to humans. AdoMet analogs with extended propargylic side chains have been chemically produced for methyltransferase-directed transfer of activated groups (mTAG) onto DNA, although the ef...

Sequence-specific methyltransferase-induced labeling of DNA (SMILing DNA).

A new concept for sequence-specific labeling of DNA by using chemically modified cofactors for DNA methyltransferases is presented. Replacement of the amino acid side chain of the natural cofactor S-adenosyl-L-methionine with an aziridine group leads to a cofactor suitable for DNA methyltransferase-catalyzed sequence-specific coupling with DNA. Sequence-specifically fluorescently labeled plasmi...

A 7-Deazaadenosylaziridine Cofactor for Sequence-Specific Labeling of DNA by the DNA Cytosine-C5 Methyltransferase M.HhaI.

DNA methyltransferases (MTases) catalyze the transfer of the activated methyl group of the cofactor S-adenosyl-l-methionine (AdoMet or SAM) to the exocyclic amino groups of adenine or cytosine or the C5 ring atom of cytosine within specific DNA sequences. The DNA adenine-N6 MTase from Thermus aquaticus (M.TaqI) is also capable of coupling synthetic N-adenosylaziridine cofactor analogues to its ...

DNMT 1 ( DNA ( cytosine - 5 - ) - methyltransferase 1 ) )

DNA ( cytosine - 5 ) - methyltransferase 1 - [ Isoform 1 ]

The murine equivalent of the human DNMT1 (DNA [cytosine-5]-methyltransferase 1; Swiss-Prot accession number: P26358) gene was deleted in ES cells via gene targeting [1]. Dnmt1-/cells possessed dramatically decreased genomic methylation and were viable; however, the mutation caused a homozygous lethal phenotype when introduced into the germline (see Table of experimental models for DNMT1) [1]. C...